Title: Development and optimisation of a PCR-based assay for study of gut microbiota-derived bile-metabolising enzymes
Clostridium difficile infection (CDI) holds a pronounced healthcare burden worldwide. The need for novel treatment options is evident: community acquired CDI incidence has risen dramatically; there has been a recent emergence of virulent strains, and there are notable limitations to current treatment. Faecal Microbiota Transplantation (FMT) is now a well recognised, cost-effective treatment for recurrent CDI. It involves the transfusion of healthy, screened donor’s faeces into the gastrointestinal tract of an infected patient. It has been shown to have great efficacy as a therapeutic modality, yet its exact pathogenesis remains unknown.
One postulation is that it restores certain gut microbiota that secrete bile-metabolising enzymes to the infected patient. These enzymes may have a vital role in C. difficile spore germination. The aim of this study was to develop and optimise a PCR-based assay for these certain bile metabolising enzymes in healthy donor stool samples. I spent time optimising this assay, trialling numerous parameters for enhanced PCR results. Now this assay can be used using faeces from a C. difficile infected patient, analysing its bilemetabolising enzyme content before and after FMT, thereby hopefully illustrating the re-establishment of microbiota-derived bile-metabolising enzymes after FMT. Eventually this will allow us to refine FMT as a therapeutic option.
Exploring the microbiota as the nexus between health and disease fascinates me; this coupled with the opportunity to work in a lab enticed me towards this particular project. Furthermore, this assay has vast potential use in a variety of other disease states where our microbiota-derived bile-metabolising enzymes are integral to disease pathogenesis.
I am absolutely thrilled to have been awarded this essay prize, and grateful to Dr Falk and Guts UK for this opportunity. I would like to thank my supervisors Professor Julian Marchesi, Dr Julie McDonald and Dr Benjamin Mullish for their ongoing support and guidance.
This award has encouraged and inspired me to develop my skills in laboratory research, and I feel motivated to further pursue a career in academic medicine within gastroenterology.Diya Kapila